activator ii cn03 Search Results


cn03  (cytoskeleton inc)
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cytoskeleton inc cn03
Selected phase portraits of VSMC fates predicted from and . (A) Phase portraits with inhibition of Rac or Rho on a stiff (25 kPa) substratum (⊿Rac = −1 and ⊿Rho = −1; top panels) or activation of Rac or Rho on a soft (2 kPa) substrate (⊿Rac = +1 and ⊿Rho = +2; bottom panels). (B) Phase portrait predicted from the activation of Rho (⊿Rho = +2) in VSMCs on a stiff (25 kPa) substrate. In (A) and (B), the black circles in the control phase portrait at 25 kPa depict the positions of the predicted contractile (solid line) and proliferative (dashed line) end states seen in VSMCs on stiff substrates. The black square in the control phase portrait at 2 kPa depicts the position of the null state predicted for VSMCs on soft substrates. The null state (square) is also seen at 25 kPa with Rac inhibition. (C) SMA reporter images and corresponding traction force images of SMA-GFP reporter VSMCs incubated on stiff (20–25 kPa) collagen-coated hydrogels with 20% FBS for 24 h and treated with <t>CN03</t> for the last 1 h. Cell scale bars, 100 μm; the traction force scale bar, 0–1,200 nN. (D) Quantification of results in (E); n ≈ 30–50 cells per condition. The dashed line shows the traction force threshold identified in that bins the cells into the high and low contractile states.
Cn03, supplied by cytoskeleton inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Selected phase portraits of VSMC fates predicted from and . (A) Phase portraits with inhibition of Rac or Rho on a stiff (25 kPa) substratum (⊿Rac = −1 and ⊿Rho = −1; top panels) or activation of Rac or Rho on a soft (2 kPa) substrate (⊿Rac = +1 and ⊿Rho = +2; bottom panels). (B) Phase portrait predicted from the activation of Rho (⊿Rho = +2) in VSMCs on a stiff (25 kPa) substrate. In (A) and (B), the black circles in the control phase portrait at 25 kPa depict the positions of the predicted contractile (solid line) and proliferative (dashed line) end states seen in VSMCs on stiff substrates. The black square in the control phase portrait at 2 kPa depicts the position of the null state predicted for VSMCs on soft substrates. The null state (square) is also seen at 25 kPa with Rac inhibition. (C) SMA reporter images and corresponding traction force images of SMA-GFP reporter VSMCs incubated on stiff (20–25 kPa) collagen-coated hydrogels with 20% FBS for 24 h and treated with CN03 for the last 1 h. Cell scale bars, 100 μm; the traction force scale bar, 0–1,200 nN. (D) Quantification of results in (E); n ≈ 30–50 cells per condition. The dashed line shows the traction force threshold identified in that bins the cells into the high and low contractile states.

Journal: Cell reports

Article Title: Mechanosensitive smooth muscle cell phenotypic plasticity emerging from a null state and the balance between Rac and Rho

doi: 10.1016/j.celrep.2021.109019

Figure Lengend Snippet: Selected phase portraits of VSMC fates predicted from and . (A) Phase portraits with inhibition of Rac or Rho on a stiff (25 kPa) substratum (⊿Rac = −1 and ⊿Rho = −1; top panels) or activation of Rac or Rho on a soft (2 kPa) substrate (⊿Rac = +1 and ⊿Rho = +2; bottom panels). (B) Phase portrait predicted from the activation of Rho (⊿Rho = +2) in VSMCs on a stiff (25 kPa) substrate. In (A) and (B), the black circles in the control phase portrait at 25 kPa depict the positions of the predicted contractile (solid line) and proliferative (dashed line) end states seen in VSMCs on stiff substrates. The black square in the control phase portrait at 2 kPa depicts the position of the null state predicted for VSMCs on soft substrates. The null state (square) is also seen at 25 kPa with Rac inhibition. (C) SMA reporter images and corresponding traction force images of SMA-GFP reporter VSMCs incubated on stiff (20–25 kPa) collagen-coated hydrogels with 20% FBS for 24 h and treated with CN03 for the last 1 h. Cell scale bars, 100 μm; the traction force scale bar, 0–1,200 nN. (D) Quantification of results in (E); n ≈ 30–50 cells per condition. The dashed line shows the traction force threshold identified in that bins the cells into the high and low contractile states.

Article Snippet: CN03 , Cytoskeleton , CN03-A.

Techniques: Inhibition, Activation Assay, Control, Incubation

KEY RESOURCES TABLE

Journal: Cell reports

Article Title: Mechanosensitive smooth muscle cell phenotypic plasticity emerging from a null state and the balance between Rac and Rho

doi: 10.1016/j.celrep.2021.109019

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: CN03 , Cytoskeleton , CN03-A.

Techniques: Recombinant, TaqMan Assay, Control, Software